miRNA Primer Design Tool

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The miRNA Design Tool is based on the UPL (Universal Probe Library) probes to design primer(s) for microRNA detection. The user gets the best result by two different Tm calculating methods. The tool designs the miRNA specific sequence of the stem-loop RT primer as well. By using the miRNA Design Tool developed by Astrid Research You have to accept to cite the results of the tests published in PLOSONE 2012 with the following title and authors*.

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*Title: A versatile method to design stem-loop primer-based quantitative PCR assays for detecting small regulatory RNA molecules
Authors: Zsolt Czimmerer¹, Hulvely Julianna², Zoltan Simandi¹, Eva Varallyay³, Zoltan Havelda³, Erzsebet Szabo¹, Attila Varga², Balazs Dezso⁴, Maria Balogh¹, Attila Horvath^1,2, Balint Domokos²,
Zsolt Torok², Laszlo Nagy^1,5, Balint L. Balint^1,6,7
Affiliations: ¹University of Debrecen, Medical and Health Science Center, Department of Biochemistry and Molecular Biology, ²Astrid Research Ltd, Debrecen,
³Agricultural Biotechnology Center, Gödöllö, ⁴University of Debrecen, Medical and Health Science Center, Institute of Pathology, ⁵Hungarian Academy of Sciences Apoptosis and Genomics Research Group, ⁶University of Debrecen, Medical and Health Science Center, Clinical Genomics Center, ⁷UD-Genomed Ltd, Debrecen